Application
Western blot analysis of SHP2-pY584 in A2058 and A375 cell line lysates (35ug/lane). SHP2 (arrow) was detected using the purified Pab.Application
Western blot analysis of SHP2 (arrow) using SHP2 Antibody (Y584) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SHP2 gene (Lane 2) (Origene Technologies).| Product Name | SHP2 Antibody (Y584) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PTPN11; PTP2C; SHPTP2; Tyrosine-protein phosphatase non-receptor type 11; Protein-tyrosine phosphatase 1D; Protein-tyrosine phosphatase 2C; SH-PTP2; SH-PTP3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:50~100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PTP2C SHPTP2 |
|---|---|
| Alternative Names | PTPN11 PTP2C SHPTP2 Tyrosine-protein phosphatase non-receptor type 11 Protein-tyrosine phosphatase 1D Protein-tyrosine phosphatase 2C SH-PTP2 SH-PTP3 |
| Molecular Weight(MW) | 68436 Da |
| Function | Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. Dephosphorylates ROCK2 at Tyr-722 resulting in stimulatation of its RhoA binding activity |
| Tissue Specificity | Widely expressed, with highest levels in heart, brain, and skeletal muscle |
| Cellular Localization | Cytoplasm. |
Application
Western blot analysis of SHP2-pY584 in A2058 and A375 cell line lysates (35ug/lane). SHP2 (arrow) was detected using the purified Pab.
Application
Western blot analysis of SHP2 (arrow) using SHP2 Antibody (Y584) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SHP2 gene (Lane 2) (Origene Technologies).| Application Notes | WB:1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
