Application
SIAH1 Antibody (Center) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the SIAH1 antibody detected the SIAH1 protein (arrow).| Product Name | SIAH1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SIAH1; HUMSIAH; E3 ubiquitin-protein ligase SIAH1; Seven in absentia homolog 1; Siah-1a |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HUMSIAH |
|---|---|
| Alternative Names | SIAH1 HUMSIAH E3 ubiquitin-protein ligase SIAH1 Seven in absentia homolog 1 Siah-1a |
| Molecular Weight(MW) | 31123 Da |
| Function | E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates E3 ubiquitin ligase activity either through direct binding to substrates or by functioning as the essential RING domain subunit of larger E3 complexes. Triggers the ubiquitin-mediated degradation of many substrates, including proteins involved in transcription regulation (ELL2, MYB, POU2AF1, PML and RBBP8), a cell surface receptor (DCC), the cell-surface receptor-type tyrosine kinase FLT3, the cytoplasmic signal transduction molecules (KLF10/TIEG1 and NUMB), an antiapoptotic protein (BAG1), a microtubule motor protein (KIF22), a protein involved in synaptic vesicle function in neurons (SYP), a structural protein (CTNNB1) and SNCAIP. Confers constitutive instability to HIPK2 through proteasomal degradation. It is thereby involved in many cellular processes such as apoptosis, tumor suppression, cell cycle, axon guidance, transcription regulation, spermatogenesis and TNF-alpha signaling. Has some overlapping function with SIAH2 Induces apoptosis in cooperation with PEG3. Upon nitric oxid (NO) generation that follows apoptotic stimulation, interacts with S- nitrosylated GAPDH, mediating the translocation of GAPDH to the nucleus. GAPDH acts as a stabilizer of SIAH1, facilitating the degradation of nuclear proteins |
| Tissue Specificity | Widely expressed at a low level. Down- regulated in advanced hepatocellular carcinomas |
| Cellular Localization | Cytoplasm. Nucleus. Note=Predominantly cytoplasmic. Partially nuclear |
| Entrez Gene | 6477 |
|---|---|
| Nucleotide Accession | NP_003022.3 NP_001006611.1 |
Application
SIAH1 Antibody (Center) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the SIAH1 antibody detected the SIAH1 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
