Application
SIAH2 Antibody (Center) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the SIAH2 antibody detected the SIAH2 protein (arrow).Application
SIAH2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SIAH2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
SIAH2 Antibody (Center) flow cytometric analysis of T47D cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | SIAH2 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SIAH2; E3 ubiquitin-protein ligase SIAH2; Seven in absentia homolog 2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | SIAH2 E3 ubiquitin-protein ligase SIAH2 Seven in absentia homolog 2 |
|---|---|
| Molecular Weight(MW) | 34615 Da |
| Function | E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates E3 ubiquitin ligase activity either through direct binding to substrates or by functioning as the essential RING domain subunit of larger E3 complexes. Triggers the ubiquitin-mediated degradation of many substrates, including proteins involved in transcription regulation (POU2AF1, PML, NCOR1), a cell surface receptor (DCC), an antiapoptotic protein (BAG1), and a protein involved in synaptic vesicle function in neurons (SYP). Mediates ubiquitination and proteasomal degradation of DYRK2 in response to hypoxia. It is thereby involved in apoptosis, tumor suppression, cell cycle, transcription and signaling processes. Has some overlapping function with SIAH1. Triggers the ubiquitin-mediated degradation of TRAF2, whereas SIAH1 can not. Promotes monoubiquitination of SNCA |
| Tissue Specificity | Widely expressed at low level. |
| Cellular Localization | Cytoplasm. Nucleus. Note=Predominantly cytoplasmic. Partially nuclear |
| Entrez Gene | 6478 |
|---|---|
| Nucleotide Accession | NP_005058.3 |
Application
SIAH2 Antibody (Center) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the SIAH2 antibody detected the SIAH2 protein (arrow).
Application
SIAH2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SIAH2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
SIAH2 Antibody (Center) flow cytometric analysis of T47D cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
