Application
The anti-Siglec5 N-term Pab is used in Western blot to detect Siglec5 in mouse liver tissue lysate.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SIGLEC5 Antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
SIGLEC5 Antibody (S23) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the SIGLEC5 antibody detected the SIGLEC5 protein (arrow).| Product Name | SIGLEC5 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SIGLEC5; CD33L2; OBBP2; Sialic acid-binding Ig-like lectin 5; CD33 antigen-like 2; Obesity-binding protein 2; CD_antigen=CD170; Flags: Precursor |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CD33L2 OBBP2 |
|---|---|
| Alternative Names | SIGLEC5 CD33L2 OBBP2 Sialic acid-binding Ig-like lectin 5 CD33 antigen-like 2 Obesity-binding protein 2 CD_antigen=CD170 Flags: Precursor |
| Molecular Weight(MW) | 60715 Da |
| Function | Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds equally to alpha-2,3-linked and alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface |
| Tissue Specificity | Expressed by monocytic/myeloid lineage cells. Found at high levels in peripheral blood leukocytes, spleen, bone marrow and at lower levels in lymph node, lung, appendix, placenta, pancreas and thymus. Expressed by monocytes and neutrophils but absent from leukemic cell lines representing early stages of myelomonocytic differentiation |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
| Entrez Gene | 8778 |
|---|
Application
The anti-Siglec5 N-term Pab is used in Western blot to detect Siglec5 in mouse liver tissue lysate.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SIGLEC5 Antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
SIGLEC5 Antibody (S23) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the SIGLEC5 antibody detected the SIGLEC5 protein (arrow).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
