Application
The anti-SPPL2b Antibody (N-term) is used in Western blot to detect SPPL2b in mouse thymus tissue lysate.Application
Western blot analysis of anti-SPPL2b Antibody (N-term) in Ramos cell line lysates (35ug/lane). SPPL2b(arrow) was detected using the purified Pab.Application
Western blot analysis of SPPL2b(arrow) using rabbit polyclonal SPPL2b Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SPPL2b gene (Lane 2) (Origene Technologies).| Product Name | Signal peptide peptidase-like 2B (SPPL2b) Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | IMP4, KIAA1532, PSL1 |
|---|---|
| Alternative Names | SPPL2B IMP4 KIAA1532 PSL1 Signal peptide peptidase-like 2B Intramembrane protease 4 Presenilin homologous protein 4 Presenilin-like protein 1 |
| Molecular Weight(MW) | 64644 Da |
| Function | Intramembrane-cleaving aspartic protease (I-CLiP) that cleaves type II membrane signal peptides in the hydrophobic plane of the membrane. Functions in ITM2B and TNF processing. Catalyzes the intramembrane cleavage of the anchored fragment of shed TNF- alpha (TNF), which promotes the release of the intracellular domain (ICD) for signaling to the nucleus. May play a role in the regulation of innate and adaptive immunity |
| Tissue Specificity | Expressed predominantly in adrenal cortex and mammary gland |
| Cellular Localization | Golgi apparatus membrane; Multi-pass membrane protein. Note=targeted through the entire secretory pathway to endosomes/lysosomes |
| Entrez Gene | 56928 |
|---|
Application
The anti-SPPL2b Antibody (N-term) is used in Western blot to detect SPPL2b in mouse thymus tissue lysate.
Application
Western blot analysis of anti-SPPL2b Antibody (N-term) in Ramos cell line lysates (35ug/lane). SPPL2b(arrow) was detected using the purified Pab.
Application
Western blot analysis of SPPL2b(arrow) using rabbit polyclonal SPPL2b Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SPPL2b gene (Lane 2) (Origene Technologies).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
