Application
SIKE Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the SIKE antibody detected the SIKE protein (arrow).Application
SIKE Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SIKE Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | SIKE Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SIKE |
|---|---|
| Alternative Names | SIKE1 SIKE Suppressor of IKBKE 1 Suppressor of IKK-epsilon |
| Molecular Weight(MW) | 23721 Da |
| Function | Physiological suppressor of IKK-epsilon and TBK1 that plays an inhibitory role in virus- and TLR3-triggered IRF3 Inhibits TLR3-mediated activation of interferon-stimulated response elements (ISRE) and the IFN-beta promoter. May act by disrupting the interactions of IKBKE or TBK1 with TICAM1/TRIF, IRF3 and DDX58/RIG-I. Does not inhibit NF-kappa-B activation pathways |
| Tissue Specificity | Widely expressed. Expressed in brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and leukocytes. Present in all cell lines tested (at protein level) |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 80143 |
|---|---|
| Nucleotide Accession | NP_001095866.1 NP_079349.2 |
Application
SIKE Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the SIKE antibody detected the SIKE protein (arrow).
Application
SIKE Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SIKE Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
