Application
Western blot analysis of SIL1 Antibody (Center) in mouse kidney tissue lysates (35ug/lane).SIL1 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with SIL1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of HepG2 cells using SIL1 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | SIL1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | SIL1 Nucleotide exchange factor SIL1 BiP-associated protein |
|---|---|
| Molecular Weight(MW) | 52085 Da |
| Function | Required for protein translocation and folding in the endoplasmic reticulum (ER). Functions as a nucleotide exchange factor for the ER lumenal chaperone HSPA5 |
| Tissue Specificity | Highly expressed in tissues which produce large amounts of secreted proteins such as kidney, liver and placenta. Also expressed in colon, heart, lung, ovary, pancreas, peripheral leukocyte, prostate, spleen and thymus. Expressed at low levels throughout the brain |
| Cellular Localization | Endoplasmic reticulum lumen. |
| Entrez Gene | 64374 |
|---|
Application
Western blot analysis of SIL1 Antibody (Center) in mouse kidney tissue lysates (35ug/lane).SIL1 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with SIL1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of HepG2 cells using SIL1 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
