Application
A. Western blot analysis on (A) SKBR-3 and (B) SH-SY5Y cell lysates using anti-SIRT2 RabMAb (Cat. #AJ1718a), dilution 1:1000.| Product Name | SIRT2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR1667 |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SIR2L, SIR2L2 |
|---|---|
| Alternative Names | SIRT2 SIR2L SIR2L2 NAD-dependent deacetylase sirtuin-2 SIR2-like protein 2 |
| Molecular Weight(MW) | 43182 Da |
| Function | NAD-dependent protein deacetylase, which deacetylates internal lysines on histone and non-histone proteins. Deacetylates 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton. Deacetylates PCK1, opposing proteasomal degradation Deacetylates 'Lys-310' of RELA |
| Tissue Specificity | Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network |
| Cellular Localization | Cytoplasm, cytoskeleton. Note=Colocalizes with microtubules |
| Entrez Gene | 22933 |
|---|
Application
A. Western blot analysis on (A) SKBR-3 and (B) SH-SY5Y cell lysates using anti-SIRT2 RabMAb (Cat. #AJ1718a), dilution 1:1000.| Application Notes | WB:1:500~1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
