Application
SKA2 Antibody (N-term) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the SKA2 antibody detected the SKA2 protein (arrow).Application
SKA2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SKA2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | SKA2 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | FAM33A |
|---|---|
| Alternative Names | SKA2 FAM33A Spindle and kinetochore-associated protein 2 Protein FAM33A |
| Molecular Weight(MW) | 14188 Da |
| Function | Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it is required for SKA1 localization |
| Tissue Specificity | This SKA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16-45 amino acids from the N-terminal region of human SKA2. |
| Cellular Localization | Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore. Note=Localizes to the outer kinetochore and spindle microtubules during mitosis in a NDC80 complex-dependent manner. Localizes to both the mitotic spindle and kinetochore-associated proteins |
| Entrez Gene | 348235 |
|---|---|
| Nucleotide Accession | NP_872426.1 |
Application
SKA2 Antibody (N-term) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the SKA2 antibody detected the SKA2 protein (arrow).
Application
SKA2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SKA2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
