Application
A.Western blot analysis on (A) 293, (B) HeLa (C) PC-12 and (D) 3T3 cell lysate using anti-SKP1 RabMAb (Cat. #AJ1719b), dilution 1:5000.Application
B. Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-SKP1 RabMAb (2617-1)| Product Name | SKP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR3303 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1000~2000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | EMC19, OCP2, SKP1A, TCEB1L |
|---|---|
| Alternative Names | SKP1 EMC19 OCP2 SKP1A TCEB1L S-phase kinase-associated protein 1 OCP-II Transcription elongation factor B |
| Molecular Weight(MW) | 18658 Da |
| Function | Essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as an adapter that links the F-box protein to CUL1. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(Cyclin F) directs ubiquitination of CP110 |
| Tissue Specificity | A synthetic peptide corresponding to residues in human SKP1 were used as an immunogen. |
| Entrez Gene | 6500 |
|---|
Application
A.Western blot analysis on (A) 293, (B) HeLa (C) PC-12 and (D) 3T3 cell lysate using anti-SKP1 RabMAb (Cat. #AJ1719b), dilution 1:5000.
Application
B. Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-SKP1 RabMAb (2617-1)| Application Notes | WB:1:1000~2000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
