Application
Western blot analysis of anti-SKP1A Pab in CEM cell line lysate (35ug/lane). SKP1A(arrow) was detected using the purified Pab (1:60 dilution).| Product Name | SKP1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | EMC19, OCP2, SKP1A, TCEB1L |
|---|---|
| Alternative Names | SKP1 EMC19 OCP2 SKP1A TCEB1L S-phase kinase-associated protein 1 Cyclin-A/CDK2-associated protein p19 Organ of Corti protein 2 Organ of Corti protein II RNA polymerase II elongation factor-like protein SIII Transcription elongation factor B p19 |
| Molecular Weight(MW) | 18658 Da |
| Function | Essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as an adapter that links the F-box protein to CUL1. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(Cyclin F) directs ubiquitination of CP110 |
| Tissue Specificity | This SKP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 65~95 amino acids from the central region of human SKP1A. |
| Entrez Gene | 6500 |
|---|
Application
Western blot analysis of anti-SKP1A Pab in CEM cell line lysate (35ug/lane). SKP1A(arrow) was detected using the purified Pab (1:60 dilution).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
