Application
SLC12A1 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the SLC12A1 antibody detected the SLC12A1 protein (arrow).Application
SLC12A1 Antibdy (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SLC12A1 Antibdy (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
SLC12A1 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | SLC12A1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | NKCC2 |
|---|---|
| Alternative Names | SLC12A1 NKCC2 Solute carrier family 12 member 1 Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2 Kidney-specific Na-K-Cl symporter |
| Molecular Weight(MW) | 121450 Da |
| Function | Electrically silent transporter system. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume |
| Tissue Specificity | Kidney specific. |
| Cellular Localization | Membrane; Multi-pass membrane protein. |
| Entrez Gene | 6557 |
|---|---|
| Nucleotide Accession | NP_000329.2 |
Application
SLC12A1 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the SLC12A1 antibody detected the SLC12A1 protein (arrow).
Application
SLC12A1 Antibdy (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SLC12A1 Antibdy (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
SLC12A1 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
