Application
Western blot analysis of SLC25A27 Antibody (Center) in Ramos cell line lysates (35ug/lane). SLC25A27 (arrow) was detected using the purified Pab;Application
Western blot analysis of SLC25A27 Antibody (Center) in mouse heart tissue lysates (35ug/lane). SLC25A27 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with SLC25A27 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
SLC25A27 Antibody (Center) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | SLC25A27 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SLC25A27; UCP4; Mitochondrial uncoupling protein 4; Solute carrier family 25 member 27 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | UCP4 |
|---|---|
| Alternative Names | SLC25A27 UCP4 Mitochondrial uncoupling protein 4 Solute carrier family 25 member 27 |
| Molecular Weight(MW) | 36064 Da |
| Function | UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat. May play a role in thermoregulatory heat production and metabolism in brain |
| Tissue Specificity | Found in adult and fetal brain. Present in most of the brain tissues, with low levels in spinal chord, corpus callosum and substantia nigra |
| Cellular Localization | Mitochondrion inner membrane; Multi-pass membrane protein |
| Entrez Gene | 9481 |
|---|
Application
Western blot analysis of SLC25A27 Antibody (Center) in Ramos cell line lysates (35ug/lane). SLC25A27 (arrow) was detected using the purified Pab;
Application
Western blot analysis of SLC25A27 Antibody (Center) in mouse heart tissue lysates (35ug/lane). SLC25A27 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with SLC25A27 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
SLC25A27 Antibody (Center) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
