Application
SLC36A1 Antibody (N-term) western blot analysis in NCI-H460(lane 1),K562(lane 2),A549(lane 3) cell line lysates (35ug/lane).This demonstrates the SLC36A1 antibody detected the SLC36A1 protein (arrow).Application
SLC36A1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human rectum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SLC36A1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of SLC36A1 Antibody (N-term)(Cat#AP12170a) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).Application
SLC36A1 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Product Name | SLC36A1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SLC36A1; PAT1; Proton-coupled amino acid transporter 1; Solute carrier family 36 member 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIFFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PAT1 |
|---|---|
| Alternative Names | SLC36A1 PAT1 Proton-coupled amino acid transporter 1 Solute carrier family 36 member 1 |
| Molecular Weight(MW) | 53076 Da |
| Function | Neutral amino acid/proton symporter. Has a pH-dependent electrogenic transport activity for small amino acids such as glycine, alanine and proline. Besides small apolar L-amino acids, it also recognize their D-enantiomers and selected amino acid derivatives such as gamma-aminobutyric acid (By similarity) |
| Tissue Specificity | This SLC36A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human SLC36A1. |
| Cellular Localization | Cell membrane; Multi-pass membrane protein (By similarity). Lysosome membrane; Multi-pass membrane protein |
| Entrez Gene | 206358 |
|---|---|
| Nucleotide Accession | NP_510968.2 |
Application
SLC36A1 Antibody (N-term) western blot analysis in NCI-H460(lane 1),K562(lane 2),A549(lane 3) cell line lysates (35ug/lane).This demonstrates the SLC36A1 antibody detected the SLC36A1 protein (arrow).
Application
SLC36A1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human rectum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SLC36A1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of SLC36A1 Antibody (N-term)(Cat#AP12170a) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Application
SLC36A1 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
