Application
SLC5A12 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the SLC5A12 antibody detected the SLC5A12 protein (arrow).Application
SLC5A12 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SLC5A12 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
SLC5A12 Antibody (C-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | SLC5A12 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SLC5A12; SMCT2; Sodium-coupled monocarboxylate transporter 2; Electroneutral sodium monocarboxylate cotransporter; Low-affinity sodium-lactate cotransporter; Solute carrier family 5 member 12 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SMCT2 |
|---|---|
| Alternative Names | SLC5A12 SMCT2 Sodium-coupled monocarboxylate transporter 2 Electroneutral sodium monocarboxylate cotransporter Low-affinity sodium-lactate cotransporter Solute carrier family 5 member 12 |
| Molecular Weight(MW) | 67647 Da |
| Function | Acts as an electroneutral and low-affinity sodium (Na(+))-dependent sodium-coupled solute transporter. Catalyzes the transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, nicotinate, propionate, butyrate and beta-D- hydroxybutyrate. May be responsible for the first step of reabsorption of monocarboxylates from the lumen of the proximal tubule of the kidney and the small intestine. May play also a role in monocarboxylates transport in the retina (By similarity) Mediates electroneutral uptake of lactate, with a stoichiometry of 2 Na(+) for each lactate (By similarity) |
| Tissue Specificity | This SLC5A12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 583-613 amino acids from the C-terminal region of human SLC5A12. |
| Cellular Localization | Apical cell membrane; Multi-pass membrane protein. Note=Detected at the brush border membrane of the kidney Colocalizes with viementin in Mueller cells (By similarity) |
| Entrez Gene | 159963 |
|---|---|
| Nucleotide Accession | NP_848593.2 |
Application
SLC5A12 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the SLC5A12 antibody detected the SLC5A12 protein (arrow).
Application
SLC5A12 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SLC5A12 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
SLC5A12 Antibody (C-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
