Application
SLC9A4 Antibody (Center) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the SLC9A4 antibody detected the SLC9A4 protein (arrow).| Product Name | SLC9A4 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SLC9A4; NHE4; Sodium/hydrogen exchanger 4; Na(+)/H(+) exchanger 4; Solute carrier family 9 member 4 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | NHE4 |
|---|---|
| Alternative Names | SLC9A4 NHE4 Sodium/hydrogen exchanger 4 Na(+)/H(+) exchanger 4 Solute carrier family 9 member 4 |
| Molecular Weight(MW) | 89814 Da |
| Function | Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction. May play a specialized role in the kidney in rectifying cell volume in response to extreme fluctuations of hyperosmolar-stimulated cell shrinkage. Is relatively amiloride and ethylisopropylamiloride (EIPA) insensitive. Can be activated under conditions of hyperosmolar-induced cell shrinkage in a sustained intracellular acidification-dependence manner. Activated by 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS) in a sustained intracellular acidification-dependence manner. Affects potassium/proton exchange as well as sodium/proton and lithium/proton exchange. In basolateral cell membrane, participates in homeostatic control of intracellular pH, and may play a role in proton extrusion in order to achieve transepithelial HCO3(-) secretion. In apical cell membrane may be involved in mediating sodium absorption. Requires for normal levels of gastric acid secretion, secretory membrane development, parietal cell maturation and/or differentiation and at least secondarily for chief cell differentiation (By similarity) |
| Tissue Specificity | This SLC9A4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 461-491 amino acids from the Central region of human SLC9A4. |
| Cellular Localization | Apical cell membrane; Multi-pass membrane protein (By similarity). Basolateral cell membrane; Multi-pass membrane protein (By similarity). Cytoplasmic granule membrane; Multi-pass membrane protein (By similarity). Note=Found in zymogen granule membranes (By similarity) |
| Entrez Gene | 389015 |
|---|---|
| Nucleotide Accession | NP_001011552.2 |
Application
SLC9A4 Antibody (Center) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the SLC9A4 antibody detected the SLC9A4 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
