Application
A. Western blot analysis on Jurkat cell lysates using anti-Phospho-SLP-76 (pY145) RabMAb (Cat. #AJ1720b), 1:200,000 dilution. Cells were either (A) untreated (B) treated with pervanadate.| Product Name | SLP-76 Antibody Phospho (pY145) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LCP2, Lymphocyte cytosolic protein 2, SH2 domain-containing leukocyte protein of 76 kDa;SLP-76 tyrosine phosphoprotein |
| Modification | p-Y145 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP2853Y |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:10000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | LCP2 Lymphocyte cytosolic protein 2 SH2 domain-containing leukocyte protein of 76 kDa SLP-76 tyrosine phosphoprotein |
|---|---|
| Molecular Weight(MW) | 60188 Da |
| Function | Involved in T-cell antigen receptor mediated signaling. |
| Tissue Specificity | Highly expressed in spleen, thymus and peripheral blood leukocytes. Highly expressed also in T-cell and monocytic cell lines, expressed at lower level in B-cell lines Not detected in fibroblast or neuroblastoma cell lines |
| Cellular Localization | Cytoplasm (Probable). |
| Entrez Gene | 3937 |
|---|
Application
A. Western blot analysis on Jurkat cell lysates using anti-Phospho-SLP-76 (pY145) RabMAb (Cat. #AJ1720b), 1:200,000 dilution. Cells were either (A) untreated (B) treated with pervanadate.| Application Notes | WB:1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
