Application
A. Western blot analysis on Hela cell lysate using anti-Smad1 RabMAb (Cat. #AJ1722a), dilution 1:2,000.| Product Name | Smad1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SMAD1, BSP1, MADH1, MADR1, Mothers against decapentaplegic homolog 1, JV4-1 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP565Y |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BSP1 MADH1 MADR1 |
|---|---|
| Alternative Names | SMAD1 BSP1 MADH1 MADR1 Mothers against decapentaplegic homolog 1 JV4-1 |
| Molecular Weight(MW) | 52260 Da |
| Function | Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1 |
| Tissue Specificity | Ubiquitous. Highest expression seen in the heart and skeletal muscle |
| Cellular Localization | Cytoplasm. Nucleus. Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane |
| Entrez Gene | 4086 |
|---|
Application
A. Western blot analysis on Hela cell lysate using anti-Smad1 RabMAb (Cat. #AJ1722a), dilution 1:2,000.| Application Notes | WB:1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
