Application
A. Western blot analysis on Jurkat cell lysate using anti-Smad2 RabMAb (Cat. #AJ1723b), dilution 1:500,000.Application
B. Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using anti-Smad2 RabMAb (Cat. #AJ1723b).| Product Name | Smad2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SMAD2, MADH2, MADR2, Mothers against decapentaplegic homolog 2, SMAD 2;hSMAD2;JV18-1 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP784Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MADH2 MADR2 |
|---|---|
| Alternative Names | SMAD2 MADH2 MADR2 Mothers against decapentaplegic homolog 2 SMAD 2 hSMAD2 JV18-1 |
| Molecular Weight(MW) | 52306 Da |
| Function | Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator |
| Tissue Specificity | Expressed at high levels in skeletal muscle, heart and placenta |
| Cellular Localization | Cytoplasm. Nucleus. Note=Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. On dephosphorylation by phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1 |
| Entrez Gene | 4087 |
|---|
Application
A. Western blot analysis on Jurkat cell lysate using anti-Smad2 RabMAb (Cat. #AJ1723b), dilution 1:500,000.
Application
B. Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using anti-Smad2 RabMAb (Cat. #AJ1723b).| Application Notes | WB:1:1000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
