Application
A. Western blot analysis on HeLa cell lysates using anti-Phospho-Smad5 (pS463/465) RabMAb (Cat. #AJ1726b), 1:500 dilution. Cells were either (A) untreated (B) treated with BMP-4Application
B. Immunofluorescent staining of HeLa cells using anti-Phospho Smad5 (pS463/465) RabMAb (Cat. #AJ1726b)| Product Name | Smad5 Antibody Phospho (pS463/465) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SMAD5, MADH5, Mothers against decapentaplegic homolog 5, hSmad5;JV5-1 |
| Modification | p-S463/465 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP728(2)AY |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:500~1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MADH5 |
|---|---|
| Alternative Names | SMAD5 MADH5 Mothers against decapentaplegic homolog 5 hSmad5 JV5-1 |
| Molecular Weight(MW) | 52258 Da |
| Function | Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD) |
| Tissue Specificity | Ubiquitous. |
| Cellular Localization | Cytoplasm. Nucleus. Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4 |
| Entrez Gene | 4090 |
|---|
Application
A. Western blot analysis on HeLa cell lysates using anti-Phospho-Smad5 (pS463/465) RabMAb (Cat. #AJ1726b), 1:500 dilution. Cells were either (A) untreated (B) treated with BMP-4
Application
B. Immunofluorescent staining of HeLa cells using anti-Phospho Smad5 (pS463/465) RabMAb (Cat. #AJ1726b)| Application Notes | WB:1:500~1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
