Application
SMAD5 Antibody (Center) pre-incubated without(lane 1) and with(lane 2) blocking peptide in Hela cell line lysate. SMAD5 Antibody (Center) (arrow) was detected using the purified Pab (1:60/250 dilution).| Product Name | SMAD5(Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SMAD5; MADH5; Mothers against decapentaplegic homolog 5; JV5-1; SMAD family member 5 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MADH5 |
|---|---|
| Alternative Names | SMAD5 MADH5 Mothers against decapentaplegic homolog 5 JV5-1 SMAD family member 5 |
| Molecular Weight(MW) | 52258 Da |
| Function | Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD) |
| Tissue Specificity | Ubiquitous. |
| Cellular Localization | Cytoplasm. Nucleus. Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4 |
| Entrez Gene | 4090 |
|---|---|
| Nucleotide Accession | NP_005894.3 |
Application
SMAD5 Antibody (Center) pre-incubated without(lane 1) and with(lane 2) blocking peptide in Hela cell line lysate. SMAD5 Antibody (Center) (arrow) was detected using the purified Pab (1:60/250 dilution).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
