Application
SMAD9 Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the SMAD9 antibody detected the SMAD9 protein (arrow).Application
Fluorescent confocal image of MCF-7 cell stained with SMAD9 Antibody (Center)(Cat#AP14903c). MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SMAD9 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).SMAD9 immunoreactivity is localized to cytoplasm and nucleus significantly.| Product Name | SMAD9 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SMAD9; MADH6; MADH9; Mothers against decapentaplegic homolog 9; Madh6; SMAD family member 9 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:100~500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MADH6 MADH9 |
|---|---|
| Alternative Names | SMAD9 MADH6 MADH9 Mothers against decapentaplegic homolog 9 Madh6 SMAD family member 9 |
| Molecular Weight(MW) | 52493 Da |
| Function | Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD9 is a receptor-regulated SMAD (R-SMAD) |
| Tissue Specificity | Expressed in heart, brain, placenta, lung, skeletal muscle, prostate, testis, ovary and small intestine. Also expressed in fetal brain, lung and kidney |
| Cellular Localization | Cytoplasm (By similarity). Nucleus (By similarity). Note=In the cytoplasm in the absence of ligand Migration to the nucleus when complexed with SMAD4 (By similarity) |
| Entrez Gene | 4093 |
|---|---|
| Nucleotide Accession | NP_005896.1 NP_001120689.1 |
Application
SMAD9 Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the SMAD9 antibody detected the SMAD9 protein (arrow).
Application
Fluorescent confocal image of MCF-7 cell stained with SMAD9 Antibody (Center)(Cat#AP14903c). MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SMAD9 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).SMAD9 immunoreactivity is localized to cytoplasm and nucleus significantly.| Application Notes | WB:1:100~500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
