Application
Smg-2 western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the Smg-2 antibody detected the Smg-2 protein (arrow).Application
Smg-2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Smg-2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Smg-2 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | Smg-2 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | smg-2; Regulator of nonsense transcripts 1; ATP-dependent helicase smg-2; Nonsense mRNA reducing factor 1; Up-frameshift suppressor 1 homolog |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | smg-2 Regulator of nonsense transcripts 1 ATP-dependent helicase smg-2 Nonsense mRNA reducing factor 1 Up-frameshift suppressor 1 homolog |
|---|---|
| Molecular Weight(MW) | 120020 Da |
| Function | RNA-dependent helicase and ATPase required for nonsense- mediated decay (NMD) of mRNAs containing premature stop codons. Is recruited to mRNAs upon translation termination and undergoes a cycle of phosphorylation and dephosphorylation; its phosphorylation appears to be a key step in NMD. The formation of an smg-2-3-4 surveillance complex is believed to activate NMD (By similarity) |
| Tissue Specificity | This Smg-2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of C. elegans Smg-2. |
| Cellular Localization | Cytoplasm (By similarity). |
| Entrez Gene | 171696 |
|---|
Application
Smg-2 western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the Smg-2 antibody detected the Smg-2 protein (arrow).
Application
Smg-2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Smg-2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Smg-2 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
