Application
SMC1B Antibody (Center) western blot analysis in human normal Uterus tissue lysates (35ug/lane).This demonstrates the SMC1B antibody detected the SMC1B protein (arrow).Application
SMC1B Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SMC1B Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | SMC1B Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SMC1B; SMC1L2; Structural maintenance of chromosomes protein 1B |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SMC1L2 |
|---|---|
| Alternative Names | SMC1B SMC1L2 Structural maintenance of chromosomes protein 1B |
| Molecular Weight(MW) | 143908 Da |
| Function | Meiosis-specific component of cohesin complex. Required for the maintenance of meiotic cohesion, but not, or only to a minor extent, for its establishment. Contributes to axial element (AE) formation and the organization of chromatin loops along the AE. Plays a key role in synapsis, recombination and chromosome movements. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The meiosis-specific cohesin complex probably replaces mitosis specific cohesin complex when it dissociates from chromatin during prophase I (By similarity) |
| Tissue Specificity | This SMC1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 736-765 amino acids from the Central region of human SMC1B. |
| Cellular Localization | Nucleus. Chromosome (By similarity). Chromosome, centromere (By similarity). Note=Associates with chromatin. In prophase I stage of meiosis, localizes along the AE of synaptonemal complexes. In late-pachytene-diplotene, the bulk of protein dissociates from the chromosome arms probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. Remains chromatin associated at the centromeres up to metaphase II. At anaphase II, dissociates from centromeres, allowing chromosomes segregation (By similarity) |
| Entrez Gene | 27127 |
|---|---|
| Nucleotide Accession | NP_683515.3 |
Application
SMC1B Antibody (Center) western blot analysis in human normal Uterus tissue lysates (35ug/lane).This demonstrates the SMC1B antibody detected the SMC1B protein (arrow).
Application
SMC1B Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SMC1B Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
