Application
A.Western blot analysis on SH-SY-5Y cell lysate using anti-Smurf2 RabMAb (Cat. #AJ1728a).| Product Name | Smurf2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SMURF2, E3 ubiquitin-protein ligase SMURF2, SMAD ubiquitination regulatory factor 2;SMAD-specific E3 ubiquitin-protein ligase 2 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP629Y3 |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000~1:10000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | SMURF2 E3 ubiquitin-protein ligase SMURF2 SMAD ubiquitination regulatory factor 2 SMAD-specific E3 ubiquitin-protein ligase 2 |
|---|---|
| Molecular Weight(MW) | 86196 Da |
| Function | E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level |
| Tissue Specificity | Widely expressed. |
| Cellular Localization | Nucleus. Cytoplasm. Cell membrane. Membrane raft. Note=Cytoplasmic in the presence of SMAD7. Co-localizes with CAV1, SMAD7 and TGF-beta receptor in membrane rafts |
| Entrez Gene | 64750 |
|---|
Application
A.Western blot analysis on SH-SY-5Y cell lysate using anti-Smurf2 RabMAb (Cat. #AJ1728a).| Application Notes | WB:1:1000~1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
