Application
SNF8 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the SNF8 antibody detected the SNF8 protein (arrow).Application
Western blot analysis of SNF8 (arrow) using rabbit polyclonal SNF8 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SNF8 gene.| Product Name | SNF8 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SNF8; EAP30; Vacuolar-sorting protein SNF8; ELL-associated protein of 30 kDa; ESCRT-II complex subunit VPS22 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | EAP30 |
|---|---|
| Alternative Names | SNF8 EAP30 Vacuolar-sorting protein SNF8 ELL-associated protein of 30 kDa ESCRT-II complex subunit VPS22 |
| Molecular Weight(MW) | 28864 Da |
| Function | Component of the endosomal sorting complex required for transport II (ESCRT-II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation by participating in derepression of transcription by RNA polymerase II, possibly via its interaction with ELL. Required for degradation of both endocytosed EGF and EGFR, but not for the EGFR ligand-mediated internalization. It is also required for the degradation of CXCR4 |
| Tissue Specificity | This SNF8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36-65 amino acids from the N-terminal region of human SNF8. |
| Cellular Localization | Cytoplasm. Endosome membrane. Nucleus (Probable). Late endosome membrane. Note=Recruited to the endosome membrane to participate in vesicle formation |
| Entrez Gene | 11267 |
|---|---|
| Nucleotide Accession | NP_009172.2 |
Application
SNF8 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the SNF8 antibody detected the SNF8 protein (arrow).
Application
Western blot analysis of SNF8 (arrow) using rabbit polyclonal SNF8 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SNF8 gene.| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
