Application
SORBS1 Antibody (Center) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the SORBS1 antibody detected the SORBS1 protein (arrow).Application
SORBS1 Antibody (Center) western blot analysis in CHO cell line lysates (35ug/lane).This demonstrates the SORBS1 antibody detected the SORBS1 protein (arrow).Application
Confocal immunofluorescent analysis of SORBS1 Antibody (Center) (Cat#AP10689c) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | SORBS1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SORBS1; KIAA0894; KIAA1296; SH3D5; Sorbin and SH3 domain-containing protein 1; Ponsin; SH3 domain protein 5; SH3P12; c-Cbl-associated protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:100~500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA0894 KIAA1296 SH3D5 |
|---|---|
| Alternative Names | SORBS1 KIAA0894 KIAA1296 SH3D5 Sorbin and SH3 domain-containing protein 1 Ponsin SH3 domain protein 5 SH3P12 c-Cbl-associated protein |
| Molecular Weight(MW) | 142513 Da |
| Function | Plays a role in tyrosine phosphorylation of CBL by linking CBL to the insulin receptor. Required for insulin- stimulated glucose transport. Involved in formation of actin stress fibers and focal adhesions (By similarity) |
| Tissue Specificity | Detected in skeletal muscle (at protein level). Widely expressed with highest levels in heart and skeletal muscle |
| Cellular Localization | Cell junction, adherens junction. Cell membrane. Cytoplasm, cytoskeleton. Cell junction, focal adhesion Nucleus (By similarity). Nucleus matrix (By similarity) Note=Colocalizes with the Ten-1 ICD form of TENM1 in the nucleus (By similarity). Colocalizes with actin stress fibers. Also detected at the plasma membrane and in neuronal intranuclear inclusions. Colocalized with PXN at focal adhesions during myogenic differentiation |
| Entrez Gene | 10580 |
|---|---|
| Nucleotide Accession | NP_006425.2 |
Application
SORBS1 Antibody (Center) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the SORBS1 antibody detected the SORBS1 protein (arrow).
Application
SORBS1 Antibody (Center) western blot analysis in CHO cell line lysates (35ug/lane).This demonstrates the SORBS1 antibody detected the SORBS1 protein (arrow).
Application
Confocal immunofluorescent analysis of SORBS1 Antibody (Center) (Cat#AP10689c) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
