Application
SORT1 Antibody (Center) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the SORT1 antibody detected the SORT1 protein (arrow).Application
SORT1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SORT1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
SORT1 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | SORT1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SORT1; Sortilin; 100 kDa NT receptor; Glycoprotein 95; Neurotensin receptor 3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | SORT1 Sortilin 100 kDa NT receptor Glycoprotein 95 Neurotensin receptor 3 |
|---|---|
| Molecular Weight(MW) | 92068 Da |
| Function | Functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface. Required for protein transport from the Golgi apparatus to the lysosomes by a pathway that is independent of the mannose-6-phosphate receptor (M6PR). Also required for protein transport from the Golgi apparatus to the endosomes. Promotes neuronal apoptosis by mediating endocytosis of the proapoptotic precursor forms of BDNF (proBDNF) and NGFB (proNGFB). Also acts as a receptor for neurotensin. May promote mineralization of the extracellular matrix during osteogenic differentiation by scavenging extracellular LPL. Probably required in adipocytes for the formation of specialized storage vesicles containing the glucose transporter SLC2A4/GLUT4 (GLUT4 storage vesicles, or GSVs). These vesicles provide a stable pool of SLC2A4 and confer increased responsiveness to insulin. May also mediate transport from the endoplasmic reticulum to the Golgi |
| Tissue Specificity | Expressed at high levels in brain, spinal cord, heart, skeletal muscle, thyroid, placenta and testis Expressed at lower levels in lymphoid organs, kidney, colon and liver |
| Cellular Localization | Membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein (Potential). Endosome membrane; Single-pass type I membrane protein (Potential). Golgi apparatus, Golgi stack membrane; Single-pass type I membrane protein (Potential). Nucleus membrane; Single-pass type I membrane protein (Potential). Cell membrane; Single-pass type I membrane protein; Extracellular side Lysosome membrane; Single-pass type I membrane protein (Potential). Note=Localized to membranes of the endoplasmic reticulum, endosomes, Golgi stack, lysosomes and nucleus. A small fraction of the protein is also localized to the plasma membrane May also be found in SLC2A4/GLUT4 storage vesicles (GSVs) in adipocytes. Localization to the plasma membrane in adipocytes may be enhanced by insulin |
| Entrez Gene | 6272 |
|---|---|
| Nucleotide Accession | NP_002950.3 |
Application
SORT1 Antibody (Center) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the SORT1 antibody detected the SORT1 protein (arrow).
Application
SORT1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SORT1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
SORT1 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
