Application
Western blot analysis of anti-SOST Antibody (N-term) Pab in mouse liver tissue lysates (35ug/lane). SOST (arrow) was detected using the purified Pab (1:60 dilution).Application
SOST Antibody (N-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the SOST antibody detected the SOST protein (arrow).Application
Western blot analysis of SOST (arrow) using rabbit polyclonal SOST Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SOST gene.Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with SOST antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | SOST Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SOST; Sclerostin |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | SOST Sclerostin |
|---|---|
| Molecular Weight(MW) | 24031 Da |
| Function | Negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation |
| Tissue Specificity | Widely expressed at low levels with highest levels in bone, cartilage, kidney, liver, bone marrow and primary osteoblasts differentiated for 21 days. Detected in the subendothelial layer of the aortic intima (at protein level) |
| Cellular Localization | Secreted, extracellular space, extracellular matrix |
| Entrez Gene | 50964 |
|---|
Application
Western blot analysis of anti-SOST Antibody (N-term) Pab in mouse liver tissue lysates (35ug/lane). SOST (arrow) was detected using the purified Pab (1:60 dilution).
Application
SOST Antibody (N-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the SOST antibody detected the SOST protein (arrow).
Application
Western blot analysis of SOST (arrow) using rabbit polyclonal SOST Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SOST gene.
Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with SOST antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
