Application
Fluorescent confocal image of SY5Y cells stained with SOX2 (C265) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP2048e SOX2 (C265) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Note the highly specific localization of the SOX2 immunosignal to the nucleus.Application
Western blot analysis of SOX2 (arrow) using rabbit polyclonal SOX2 Antibody (C265) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SOX2 gene (Lane 2) (Origene Technologies).Application
SOX2 Antibody (C265) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | SOX2 Antibody (C265) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SOX2; Transcription factor SOX-2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFWBFC |
| IF:1:100 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | SOX2 Transcription factor SOX-2 |
|---|---|
| Molecular Weight(MW) | 34310 Da |
| Function | Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity). Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity) |
| Tissue Specificity | This SOX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 260~289 amino acids surrounding C265 of human SOX2. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 6657 |
|---|
Application
Fluorescent confocal image of SY5Y cells stained with SOX2 (C265) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP2048e SOX2 (C265) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Note the highly specific localization of the SOX2 immunosignal to the nucleus.
Application
Western blot analysis of SOX2 (arrow) using rabbit polyclonal SOX2 Antibody (C265) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SOX2 gene (Lane 2) (Origene Technologies).
Application
SOX2 Antibody (C265) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | IF:1:100 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
