Application
Western blot analysis of SOX4 antibody (N-term) in A2058 cell line lysates (35ug/lane). SOX4 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human brain reacted with SOX4 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
SOX4 Antibody (N-term) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Fluorescent confocal image of Hela cell stained with SOX4 Antibody (N-term)(Cat#AP2045a).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SOX4 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). SOX4 immunoreactivity is localized to Mitochondria significantly and Nucleus weakly.| Product Name | SOX4 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SOX4; Transcription factor SOX-4 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFCIF |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | SOX4 Transcription factor SOX-4 |
|---|---|
| Molecular Weight(MW) | 47263 Da |
| Function | Transcriptional activator that binds with high affinity to the T-cell enhancer motif 5'-AACAAAG-3' motif |
| Tissue Specificity | Testis, brain, and heart. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 6659 |
|---|
Application
Western blot analysis of SOX4 antibody (N-term) in A2058 cell line lysates (35ug/lane). SOX4 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human brain reacted with SOX4 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
SOX4 Antibody (N-term) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Fluorescent confocal image of Hela cell stained with SOX4 Antibody (N-term)(Cat#AP2045a).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SOX4 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). SOX4 immunoreactivity is localized to Mitochondria significantly and Nucleus weakly.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
