Application
SP1 Antibody (C-term P692) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the SP1 antibody detected the SP1 protein (arrow).Application
SP1 Antibody (C-term P692) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SP1 Antibody (C-term P692) for immunohistochemistry. Clinical relevance has not been evaluated.Application
SP1 Antibody (C-term P692) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of SP1 Antibody (C-term P692)(Cat#AP11451b) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | SP1 Antibody (C-term P692) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SP1; TSFP1; Transcription factor Sp1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFCIF |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | TSFP1 |
|---|---|
| Alternative Names | SP1 TSFP1 Transcription factor Sp1 |
| Molecular Weight(MW) | 80693 Da |
| Function | Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR- alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter Plays an essential role in the regulation of FE65 gene expression In complex with ATF7IP, maintains telomerase activity in cancer cells by inducing TERT and TERC gene expression |
| Tissue Specificity | Up-regulated in adenocarcinomas of the stomach (at protein level) |
| Cellular Localization | Nucleus. Cytoplasm. Note=Nuclear location is governed by glycosylated/phosphorylated states. Insulin promotes nuclear location, while glucagon favors cytoplasmic location |
| Entrez Gene | 6667 |
|---|---|
| Nucleotide Accession | NP_612482.2 |
Application
SP1 Antibody (C-term P692) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the SP1 antibody detected the SP1 protein (arrow).
Application
SP1 Antibody (C-term P692) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SP1 Antibody (C-term P692) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
SP1 Antibody (C-term P692) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of SP1 Antibody (C-term P692)(Cat#AP11451b) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
