Application
Western blot analysis of SP110 antibody (Center) in HL60 cell line lysates (35ug/lane). SP110 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human lymph tissue reacted with SP110 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of widr cells using SP110 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of SP110 Antibody (Center) with Hela cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | SP110 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SP110; Sp110 nuclear body protein; Interferon-induced protein 41/75; Speckled 110 kDa; Transcriptional coactivator Sp110 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFCIF |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | SP110 Sp110 nuclear body protein Interferon-induced protein 41/75 Speckled 110 kDa Transcriptional coactivator Sp110 |
|---|---|
| Molecular Weight(MW) | 78396 Da |
| Function | Transcription factor. May be a nuclear hormone receptor coactivator. Enhances transcription of genes with retinoic acid response elements (RARE) |
| Tissue Specificity | Highly expressed in peripheral blood leukocytes and spleen. Detected at intermediate levels in thymus, prostate, testis, ovary, small intestine and colon, and at low levels in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas |
| Cellular Localization | Nucleus. Note=Found in the nuclear body. |
| Entrez Gene | 3431 |
|---|
Application
Western blot analysis of SP110 antibody (Center) in HL60 cell line lysates (35ug/lane). SP110 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human lymph tissue reacted with SP110 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of widr cells using SP110 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of SP110 Antibody (Center) with Hela cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
