Application
SP140 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the SP140 antibody detected the SP140 protein (arrow).Application
SP140 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SP140 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Fluorescent confocal image of Hela cell stained with SP140 Antibody (Center)(Cat#AP12872c).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SP140 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). SP140 immunoreactivity is localized to Cytoplasm and Nucleus significantly.| Product Name | SP140 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SP140; LYSP100; Nuclear body protein SP140; Lymphoid-restricted homolog of Sp100; Nuclear autoantigen Sp-140; Speckled 140 kDa |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | LYSP100 |
|---|---|
| Alternative Names | SP140 LYSP100 Nuclear body protein SP140 Lymphoid-restricted homolog of Sp100 Nuclear autoantigen Sp-140 Speckled 140 kDa |
| Molecular Weight(MW) | 98223 Da |
| Function | Component of the nuclear body, also known as nuclear domain 10, PML oncogenic domain, and KR body. May be involved in the pathogenesis of acute promyelocytic leukemia and viral infection |
| Tissue Specificity | High levels in spleen and peripheral blood leukocytes, much lower levels in thymus, prostate, ovary, small intestine, and colon. Very low levels in heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas |
| Cellular Localization | Nucleus. Cytoplasm. Note=Localized to nuclear structures termed LANDS, for LYSp100-associated nuclear domains. LANDS are globular, electron-dense structures most often found in the nucleoplasm, but also found at the nuclear membrane and in the cytoplasm, suggesting that these structures may traffic between the cytoplasm and the nucleus |
| Entrez Gene | 11262 |
|---|---|
| Nucleotide Accession | NP_009168.4 |
Application
SP140 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the SP140 antibody detected the SP140 protein (arrow).
Application
SP140 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SP140 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Fluorescent confocal image of Hela cell stained with SP140 Antibody (Center)(Cat#AP12872c).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SP140 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). SP140 immunoreactivity is localized to Cytoplasm and Nucleus significantly.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
