Application
SPAM1 Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the SPAM1 antibody detected the SPAM1 protein (arrow).| Product Name | SPAM1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SPAM1; HYAL3; PH20; Hyaluronidase PH-20; Hyaluronoglucosaminidase PH-20; Sperm adhesion molecule 1; Sperm surface protein PH-20 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HYAL3 PH20 |
|---|---|
| Alternative Names | SPAM1 HYAL3 PH20 Hyaluronidase PH-20 Hyaluronoglucosaminidase PH-20 Sperm adhesion molecule 1 Sperm surface protein PH-20 |
| Molecular Weight(MW) | 57848 Da |
| Function | Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid |
| Tissue Specificity | Testis. |
| Cellular Localization | Cell membrane; Lipid-anchor, GPI-anchor. |
| Entrez Gene | 6677 |
|---|---|
| Nucleotide Accession | NP_694859.1 |
Application
SPAM1 Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the SPAM1 antibody detected the SPAM1 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
