Application
SPATA18 Antibody (N-term) western blot analysis in NCI-H292 cell line lysates (15ug/lane).This demonstrates the SPATA18 antibody detected SPATA18 protein (arrow).Application
SPATA18 Antibody (N-term) western blot analysis in mouse stomach tissue lysates (15ug/lane).This demonstrates the SPATA18 antibody detected SPATA18 protein (arrow).Application
SPATA18 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SPATA18 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | SPATA18 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SPATA18; MIEAP; Mitochondria-eating protein; Spermatogenesis-associated protein 18 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MIEAP |
|---|---|
| Alternative Names | SPATA18 MIEAP Mitochondria-eating protein Spermatogenesis-associated protein 18 |
| Molecular Weight(MW) | 61109 Da |
| Function | Key regulator of mitochondrial quality that mediates the repairing or degradation of unhealthy mitochondria in response to mitochondrial damage. Mediator of mitochondrial protein catabolic process (also named MALM) by mediating the degradation of damaged proteins inside mitochondria by promoting the accumulation in the mitochondrial matrix of hydrolases that are characteristic of the lysosomal lumen. Also involved in mitochondrion degradation of damaged mitochondria by promoting the formation of vacuole-like structures (named MIV), which engulf and degrade unhealthy mitochondria by accumulating lysosomes. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix |
| Tissue Specificity | This SPATA18 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 91-120 amino acids from the N-terminal region of human SPATA18. |
| Cellular Localization | Cytoplasm. Mitochondrion outer membrane. Note=Localizes to the cytoplasm under normal conditions Relocalizes to mitochondrion outer membrane following cellular stress. Colocalizes with BNIP3 and BNIP3L at the mitochondrion outer membrane |
| Entrez Gene | 132671 |
|---|---|
| Nucleotide Accession | NP_660306.1 |
Application
SPATA18 Antibody (N-term) western blot analysis in NCI-H292 cell line lysates (15ug/lane).This demonstrates the SPATA18 antibody detected SPATA18 protein (arrow).
Application
SPATA18 Antibody (N-term) western blot analysis in mouse stomach tissue lysates (15ug/lane).This demonstrates the SPATA18 antibody detected SPATA18 protein (arrow).
Application
SPATA18 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SPATA18 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
