Application
Western blot analysis of SPB3 antibody (N-term) in 293 cell line lysates (35ug/lane). SPB3 (arrow) was detected using the purified Pab.Application
SPB3 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SPB3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
SPB3 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | SPB3 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SERPINB3; SCCA; SCCA1; Serpin B3; Protein T4-A; Squamous cell carcinoma antigen 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SCCA SCCA1 |
|---|---|
| Alternative Names | SERPINB3 SCCA SCCA1 Serpin B3 Protein T4-A Squamous cell carcinoma antigen 1 |
| Molecular Weight(MW) | 44565 Da |
| Function | May act as a protease inhibitor to modulate the host immune response against tumor cells |
| Tissue Specificity | Squamous cells. Expressed in some hepatocellular carcinoma (at protein level) |
| Cellular Localization | Cytoplasm. Note=Seems to also be secreted in plasma by cancerous cells but at a low level |
| Entrez Gene | 6317 |
|---|
Application
Western blot analysis of SPB3 antibody (N-term) in 293 cell line lysates (35ug/lane). SPB3 (arrow) was detected using the purified Pab.
Application
SPB3 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SPB3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
SPB3 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
