Application
SPC25 Antibody (Center) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the SPC25 antibody detected the SPC25 protein (arrow).| Product Name | SPC25 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SPC25; SPBC25; Kinetochore protein Spc25 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SPBC25 |
|---|---|
| Alternative Names | SPC25 SPBC25 Kinetochore protein Spc25 |
| Molecular Weight(MW) | 26153 Da |
| Function | Acts as a component of the essential kinetochore- associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore |
| Tissue Specificity | This SPC25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 86-114 amino acids from the Central region of human SPC25. |
| Cellular Localization | Nucleus. Chromosome, centromere, kinetochore. Note=Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore |
| Entrez Gene | 57405 |
|---|---|
| Nucleotide Accession | NP_065726.1 |
Application
SPC25 Antibody (Center) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the SPC25 antibody detected the SPC25 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
