Application
Western blot analysis of SPCS3 antibody (C-term) in mouse liver tissue lysates (35ug/lane). SPCS3 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with SPCS3 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
SPCS3 Antibody (C-term) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | SPCS3 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SPCS3; SPC22; Signal peptidase complex subunit 3; Microsomal signal peptidase 22/23 kDa subunit |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SPC22 |
|---|---|
| Alternative Names | SPCS3 SPC22 Signal peptidase complex subunit 3 Microsomal signal peptidase 22/23 kDa subunit |
| Molecular Weight(MW) | 20313 Da |
| Function | Component of the microsomal signal peptidase complex which removes signal peptides from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum (By similarity) |
| Tissue Specificity | This SPCS3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 151~180 amino acids from the C-terminal region of human SPCS3. |
| Cellular Localization | Microsome membrane; Single-pass type II membrane protein (By similarity). Endoplasmic reticulum membrane; Single-pass type II membrane protein (By similarity) |
| Entrez Gene | 60559 |
|---|
Application
Western blot analysis of SPCS3 antibody (C-term) in mouse liver tissue lysates (35ug/lane). SPCS3 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with SPCS3 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
SPCS3 Antibody (C-term) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
