Application
Western blot analysis of SPDYA Antibody (Center) in K562 cell line lysates (35ug/lane). SPDYA (arrow) was detected using the purified Pab.Application
SPDYA Antibody (Center) IHC analysis in formalin fixed and paraffin embedded prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SPDYA Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
SPDYA Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of SPDYA (arrow) using rabbit polyclonal SPDYA Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SPDYA gene.| Product Name | SPDYA Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SPDYA; SPDY1; SPY1; Speedy protein A; Rapid inducer of G2/M progression in oocytes A; Speedy-1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SPDY1 SPY1 |
|---|---|
| Alternative Names | SPDYA SPDY1 SPY1 Speedy protein A Rapid inducer of G2/M progression in oocytes A Speedy-1 |
| Molecular Weight(MW) | 36463 Da |
| Function | Regulates the G1/S phase transition of the cell cycle by binding and activating CDK1, CDK2 and CDKN1B/KIP1. Can activate CDK2 without promoting CDK2 phosphorylation. Mediates cell survival during the DNA damage process through activation of CDK2 |
| Tissue Specificity | Highly expressed in testis. Expressed at a low level in wide range of tissues including bone marrow, brain, heart, kidney, colon, liver, placenta, spleen, skeletal muscle, salivary gland, thyroid gland, thymus, trachea and uterus Expressed at a slightly higher level in adrenal gland, cerebellum, small intestine, lung, prostate and trachea. Expression is cell cycle-dependent, being restricted to cells in G1/S phase |
| Cellular Localization | Nucleus. |
| Entrez Gene | 245711 |
|---|
Application
Western blot analysis of SPDYA Antibody (Center) in K562 cell line lysates (35ug/lane). SPDYA (arrow) was detected using the purified Pab.
Application
SPDYA Antibody (Center) IHC analysis in formalin fixed and paraffin embedded prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SPDYA Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
SPDYA Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of SPDYA (arrow) using rabbit polyclonal SPDYA Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SPDYA gene.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
