Application
SPECC1 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the SPECC1 antibody detected the SPECC1 protein (arrow).Application
SPECC1 Antibody (Center) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the SPECC1 antibody detected the SPECC1 protein (arrow).Application
SPECC1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SPECC1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
SPECC1 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of SPECC1 Antibody (Center)(Cat#AP12147c) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).| Product Name | SPECC1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SPECC1L; CYTSA; KIAA0376; Cytospin-A; Renal carcinoma antigen NY-REN-22; Sperm antigen with calponin homology and coiled-coil domains 1-like |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFCIF |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CYTSA KIAA0376 |
|---|---|
| Alternative Names | SPECC1L CYTSA KIAA0376 Cytospin-A Renal carcinoma antigen NY-REN-22 Sperm antigen with calponin homology and coiled-coil domains 1-like |
| Molecular Weight(MW) | 124602 Da |
| Function | Involved in cytokinesis and spindle organization. May play a role in actin cytoskeleton organization and microtubule stabilization and hence required for proper cell adhesion and migration |
| Tissue Specificity | This SPECC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 712-741 amino acids from the Central region of human SPECC1. |
| Cellular Localization | Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, spindle. Cell junction, gap junction Note=Colocalizes with acetylated alpha-tubulin, gamma-tubulin and F-actin. Also observed in a ring around gamma-tubulin containing centrioles possibly in the microtubule organizing center |
| Entrez Gene | 23384 |
|---|---|
| Nucleotide Accession | NP_001138940.1 |
Application
SPECC1 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the SPECC1 antibody detected the SPECC1 protein (arrow).
Application
SPECC1 Antibody (Center) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the SPECC1 antibody detected the SPECC1 protein (arrow).
Application
SPECC1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SPECC1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
SPECC1 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of SPECC1 Antibody (Center)(Cat#AP12147c) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
