Application
SPIC Antibody (Center) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the EKI2 antibody detected the EKI2 protein (arrow).Application
SPIC Antibody (Center) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Fluorescent confocal image of A431 cell stained with SPIC Antibody (Center)(Cat#AP10278c).A431 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SPIC primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). SPIC immunoreactivity is localized to Cytoplasm and Nucleus significantly.| Product Name | SPIC Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SPIC; Transcription factor Spi-C |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFCIF |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | SPIC Transcription factor Spi-C |
|---|---|
| Molecular Weight(MW) | 29180 Da |
| Function | Controls the development of red pulp macrophages required for red blood cells recycling and iron homeostasis Transcription factor that binds to the PU-box, a purine-rich DNA sequence (5'-GAGGA[AT]-3') that can act as a lymphoid-specific enhancer. Regulates VCAM1 gene expression (By similarity) |
| Tissue Specificity | Preferentially detected in fetal and adult spleen, lymph nodes and at lower levels in bone marrow and fetal liver |
| Cellular Localization | Nucleus (Probable). |
| Entrez Gene | 121599 |
|---|---|
| Nucleotide Accession | NP_689536.1 |
Application
SPIC Antibody (Center) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the EKI2 antibody detected the EKI2 protein (arrow).
Application
SPIC Antibody (Center) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Fluorescent confocal image of A431 cell stained with SPIC Antibody (Center)(Cat#AP10278c).A431 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SPIC primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). SPIC immunoreactivity is localized to Cytoplasm and Nucleus significantly.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
