Application
Western blot analysis of SPINK5 Antibody (N-term) in A2058 cell line lysates (35ug/lane). SPINK5 (arrow) was detected using the purified Pab.Application
SPINK5 Antibody (N-term) (RB18954) IHC analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SPINK5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
SPINK5 Antibody (N-term) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | SPINK5 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SPINK5; Serine protease inhibitor Kazal-type 5; Lympho-epithelial Kazal-type-related inhibitor; Hemofiltrate peptide HF6478; Hemofiltrate peptide HF7665 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | SPINK5 Serine protease inhibitor Kazal-type 5 Lympho-epithelial Kazal-type-related inhibitor Hemofiltrate peptide HF6478 Hemofiltrate peptide HF7665 |
|---|---|
| Molecular Weight(MW) | 120714 Da |
| Function | Serine protease inhibitor, probably important for the anti-inflammatory and/or antimicrobial protection of mucous epithelia. Contribute to the integrity and protective barrier function of the skin by regulating the activity of defense- activating and desquamation-involved proteases. Inhibits KLK5, it's major target, in a pH-dependent manner. Inhibits KLK7, KLK14 CASP14, and trypsin |
| Tissue Specificity | Highly expressed in the thymus and stratum corneum. Also found in the oral mucosa, parathyroid gland, Bartholin's glands, tonsils, and vaginal epithelium. Very low levels are detected in lung, kidney, and prostate |
| Cellular Localization | Secreted. |
| Entrez Gene | 11005 |
|---|
Application
Western blot analysis of SPINK5 Antibody (N-term) in A2058 cell line lysates (35ug/lane). SPINK5 (arrow) was detected using the purified Pab.
Application
SPINK5 Antibody (N-term) (RB18954) IHC analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SPINK5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
SPINK5 Antibody (N-term) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
