Application
SPI1 Antibody (C-term) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the SPI1 antibody detected the SPI1 protein (arrow).Application
SPI1 Antibody (C-term) (AP14850b)immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SPI1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | SPI1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SPI1; Transcription factor PU.1; 31 kDa-transforming protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | SPI1 Transcription factor PU.1 31 kDa-transforming protein |
|---|---|
| Molecular Weight(MW) | 31083 Da |
| Function | Binds to the PU-box, a purine-rich DNA sequence (5'- GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B- cells. Also binds RNA and may modulate pre-mRNA splicing (By similarity) |
| Tissue Specificity | This SPI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 216-246 amino acids from the C-terminal region of human SPI1. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 6688 |
|---|---|
| Nucleotide Accession | NP_003111.2 NP_001074016.1 |
Application
SPI1 Antibody (C-term) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the SPI1 antibody detected the SPI1 protein (arrow).
Application
SPI1 Antibody (C-term) (AP14850b)immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SPI1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
