Application
SPN/CD43 Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the SPN/CD43 antibody detected the SPN/CD43 protein (arrow).Application
SPN/CD43 Antibody (Center) (AP14085c)immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SPN/CD43 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | SPN/CD43 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SPN; CD43; Leukosialin; Galactoglycoprotein; Leukocyte sialoglycoprotein; Sialophorin; CD_antigen=CD43; Flags: Precursor |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CD43 |
|---|---|
| Alternative Names | SPN CD43 Leukosialin Galactoglycoprotein Leukocyte sialoglycoprotein Sialophorin CD_antigen=CD43 Flags: Precursor |
| Molecular Weight(MW) | 40322 Da |
| Function | One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter-receptor for SN/Siglec-1 (By similarity). During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response (By similarity) |
| Tissue Specificity | Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
| Entrez Gene | 6693 |
|---|---|
| Nucleotide Accession | NP_001025459.1 NP_003114.1 |
Application
SPN/CD43 Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the SPN/CD43 antibody detected the SPN/CD43 protein (arrow).
Application
SPN/CD43 Antibody (Center) (AP14085c)immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SPN/CD43 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
