Application
The anti-SPPL2a Pab is used in Western blot to detect SPPL2a in Jurkat cell lysate.Application
Formalin-fixed and paraffin-embedded placenta tissue reacted with SPPL2a Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | SPPL2a Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SPPL2A; IMP3; PSL2; Signal peptide peptidase-like 2A; Intramembrane protease 3; Presenilin-like protein 2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | IMP3 PSL2 |
|---|---|
| Alternative Names | SPPL2A IMP3 PSL2 Signal peptide peptidase-like 2A Intramembrane protease 3 Presenilin-like protein 2 |
| Molecular Weight(MW) | 58143 Da |
| Function | Intramembrane-cleaving aspartic protease (I-CLiP) that cleaves type II membrane signal peptides in the hydrophobic plane of the membrane. Functions in FASLG, ITM2B and TNF processing Catalyzes the intramembrane cleavage of the anchored fragment of shed TNF-alpha (TNF), which promotes the release of the intracellular domain (ICD) for signaling to the nucleus. Also responsible for the intramembrane cleavage of Fas antigen ligand FASLG, which promotes the release of the intracellular FasL domain (FasL ICD). May play a role in the regulation of innate and adaptive immunity |
| Tissue Specificity | Ubiquitous. |
| Cellular Localization | Late endosome membrane; Multi-pass membrane protein (By similarity). Lysosome membrane; Multi-pass membrane protein (By similarity). Cell membrane. Note=Colocalizes with palmitoylated and myristoylated proteins at the plasma membrane |
| Entrez Gene | 84888 |
|---|
Application
The anti-SPPL2a Pab is used in Western blot to detect SPPL2a in Jurkat cell lysate.
Application
Formalin-fixed and paraffin-embedded placenta tissue reacted with SPPL2a Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
