Application
Western blot analysis of IMP-3 Antibody (Center) in K562 cell line lysates (35ug/lane). IMP-3 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human brain reacted with IMP-3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of K562 cells using IMP-3 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | IMP-3 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IGF2BP3; IMP3; KOC1; VICKZ3; Insulin-like growth factor 2 mRNA-binding protein 3; IGF-II mRNA-binding protein 3; KH domain-containing protein overexpressed in cancer; VICKZ family member 3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | IMP3 KOC1 VICKZ3 |
|---|---|
| Alternative Names | IGF2BP3 IMP3 KOC1 VICKZ3 Insulin-like growth factor 2 mRNA-binding protein 3 IGF-II mRNA-binding protein 3 KH domain-containing protein overexpressed in cancer VICKZ family member 3 |
| Molecular Weight(MW) | 63705 Da |
| Function | RNA-binding protein that act as a regulator of mRNA translation and stability. Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binds to sequences in the 3'-UTR of CD44 mRNA |
| Tissue Specificity | Expressed in fetal liver, fetal lung, fetal kidney, fetal thymus, fetal placenta, fetal follicles of ovary and gonocytes of testis, growing oocytes, spermatogonia and semen (at protein level). Expressed in cervix adenocarcinoma, in testicular, pancreatic and renal-cell carcinomas (at protein level). Expressed ubiquitously during fetal development at 8 and 14 weeks of gestation. Expressed in ovary, testis, brain, placenta, pancreatic cancer tissues and pancreatic cancer cell lines |
| Cellular Localization | Nucleus. Cytoplasm. Note=Found in lamellipodia of the leading edge, in the perinuclear region, and beneath the plasma membrane. The subcytoplasmic localization is cell specific and regulated by cell contact and growth. Localized at the connecting piece and the tail of the spermatozoa Colocalized with CD44 mRNA in RNP granules |
| Entrez Gene | 10643 |
|---|
Application
Western blot analysis of IMP-3 Antibody (Center) in K562 cell line lysates (35ug/lane). IMP-3 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human brain reacted with IMP-3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of K562 cells using IMP-3 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
