Application
Western blot analysis of Phospho-SPRY4-Y75.ctrl in K562 cell line lysates (35ug/lane). SPRY4 (arrow) was detected using the purified Pab.Application
SPRY4-Y75 Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SPRY4-Y75 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.Application
SPRY4-Y75 Antibody flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | SPRY4-Y75 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SPRY4; Protein sprouty homolog 4 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | SPRY4 Protein sprouty homolog 4 |
|---|---|
| Molecular Weight(MW) | 32541 Da |
| Function | Suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras. Probably impairs the formation of GTP-Ras. Inhibits Ras-independent, but not Ras-dependent, activation of RAF1 |
| Tissue Specificity | This SPRY4-Y75 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 57~86 amino acids from the Y75 region of human SPRY4. |
| Cellular Localization | Cytoplasm. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side (Potential) |
| Entrez Gene | 81848 |
|---|
Application
Western blot analysis of Phospho-SPRY4-Y75.ctrl in K562 cell line lysates (35ug/lane). SPRY4 (arrow) was detected using the purified Pab.
Application
SPRY4-Y75 Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SPRY4-Y75 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Application
SPRY4-Y75 Antibody flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
