Application
SPT13 Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the SPT13 antibody detected the SPT13 protein (arrow).Application
SPT13 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SPT13 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
SPT13 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | SPT13 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SPATA13; Spermatogenesis-associated protein 13; APC-stimulated guanine nucleotide exchange factor 2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | SPATA13 Spermatogenesis-associated protein 13 APC-stimulated guanine nucleotide exchange factor 2 |
|---|---|
| Molecular Weight(MW) | 74820 Da |
| Function | Acts as guanine nucleotide exchange factor (GEF) for RHOA, RAC1 and CDC42 GTPases. Regulates cell migration and adhesion assembly and disassembly through a RAC1, PI3K, RHOA and AKT1-dependent mechanism. Increases both RAC1 and CDC42 activity, but decreases the amount of active RHOA. Required for MMP9 up- regulation via the JNK signaling pathway in colorectal tumor cells. Involved in tumor angiogenesis and may play a role in intestinal adenoma formation and tumor progression |
| Tissue Specificity | Expressed at high levels in the placenta, spleen and kidney, at moderate levels in lung, small intestine, liver, brain and heart, and at low levels in skeletal muscle Expression is aberrantly enhanced in most colorectal tumors |
| Cellular Localization | Cytoplasm. Cell projection, filopodium. Cell projection, lamellipodium. Cell projection, ruffle membrane Note=Accumulates in the lamellipodium and ruffle membrane in response to hepatocyte growth factor (HGF) treatment |
| Entrez Gene | 221178 |
|---|---|
| Nucleotide Accession | NP_694568.1 |
Application
SPT13 Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the SPT13 antibody detected the SPT13 protein (arrow).
Application
SPT13 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SPT13 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
SPT13 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
