Application
Western blot analysis of anti-SPTLC1 Antibody (C-term) in 293 cell line lysates (35ug/lane).SPTLC1(arrow) was detected using the purified Pab.| Product Name | SPTLC1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SPTLC1; LCB1; Serine palmitoyltransferase 1; Long chain base biosynthesis protein 1; Serine-palmitoyl-CoA transferase 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | LCB1 |
|---|---|
| Alternative Names | SPTLC1 LCB1 Serine palmitoyltransferase 1 Long chain base biosynthesis protein 1 Serine-palmitoyl-CoA transferase 1 |
| Molecular Weight(MW) | 52744 Da |
| Function | Serine palmitoyltransferase (SPT). The heterodimer formed with SPTLC2 or SPTLC3 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates, with a slight preference for C14-CoA. The SPTLC1- SPTLC2-SPTSSB complex shows a strong preference for C18-CoA substrate, while the SPTLC1-SPTLC3-SPTSSB isozyme displays an ability to use a broader range of acyl-CoAs, without apparent preference |
| Tissue Specificity | Widely expressed. Not detected in small intestine |
| Cellular Localization | Endoplasmic reticulum membrane; Single-pass membrane protein (By similarity) |
| Entrez Gene | 10558 |
|---|
Application
Western blot analysis of anti-SPTLC1 Antibody (C-term) in 293 cell line lysates (35ug/lane).SPTLC1(arrow) was detected using the purified Pab.| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
